Biological sources of variations of tartrate-resistant acid phosphatase 5b in a healthy Japanese population

High Uric Acid (UA) Negatively Affects Serum Tartrate-Resistant Acid Phosphatase 5b (TRACP 5b) Immunoassay

BACKGROUND Bone metastases often occur in the majority of patients with advanced cancer, such as prostate cancer, lung cancer and breast cancer. Serum tartrate-resistant acid phosphatase 5b (TRACP 5b), a novel bone resorption marker, has been used gradually in the clinics as a specific and sensitive marker of bone resorption for the early diagnosis of cancer patients with bone metastasis. Here,...

Development of a kinetic assay for band 5b tartrate-resistant acid phosphatase activity in serum.

BACKGROUND Band 5 tartrate-resistant acid phosphatase (TrACP; EC 3. 1.3.2) consists of two isoenzymes, bands 5a and 5b, of which band 5b TrACP is considered to be derived from bone. However, no kinetic method for the specific measurement of band 5b TrACP in serum is available. Our aim was to develop a kinetic assay method for the specific measurement of band 5b TrACP in serum. METHODS Band 5b...

Serum tartrate-resistant acid phosphatase 5b is a specific and sensitive marker of bone resorption.

ϭ the number of measurements for each calibration point x ៮ ϭ the mean values of all calibrator concentrations The resulting detection limits for normal-and low-range applications are given in Table 1. The assays were linear up to 65 mg/L for Apo A-II and 8 mg/L for Apo C-III and ApoE. For ApoA-I and ApoB, a high-dose hook effect attributable to antigen excess was observed starting at 275 mg/L ...

Biogenesis of tartrate-resistant acid phosphatase isoforms 5a and 5b in cell and animal models

Evaluation of tartrate-resistant acid phosphatase (TRACP) 5b as bone resorption marker in irradiated bone metastases.

BACKGROUND The aim of our study was to evaluate if the determination of the active isoform 5b of tartrate-resistant acid phosphatase (TRACP 5b) provides the possibility to monitor the effect of local radiotherapy in bone metastases and if TRACP 5b will predict further osseous progression. MATERIALS AND METHODS In 48 breast cancer patients with bone metastases, patients' characteristics, diagn...